The mapping of coordinates takes the form x = u + Δ u. Please read the ImageJ documentation to learn how to install plugins.įour types of transformation are considered: Other relevant on-line publications are available at. Unser, " A Pyramid Approach to Subpixel Registration Based on Intensity," IEEE Transactions on Image Processing, vol. This set of Java classes is based on the following paper: It includes the complete set of source files, along with the precompiled classes. In case of doubt, just try no harm will ensue.) (Please note that an RGB-stack is a stack of three color components it should not be confounded with a stack of RGB-color images: the latter is indeed admissible, while the former is not. The name and the type of the stack is left unchanged all data types are admissible, except RGB-stack and HSB-stack. This ancillary plugin is available here.Īfter completion of the registration process, the original stack is destroyed and replaced by the result of the registration. The StackReg plugin requires that a second plugin, named TurboReg, is installed. When the plugin is launched, the current slice acts as a global anchor. In turn, each slice is used as the template with respect to which the next slice is aligned, so that the alignment proceeds by propagation. The purpose of this plugin is to register-in other words, to align or to match-a stack of image slices. The ninth slice was used as anchor slice. Right: Stack after having applied the alignment procedure. The sections are 0.84mm apart and the pixel size is (0.126mm × 0.126mm). Left: Set of Nissl-stained sections of a murine brain. Philippe Thévenaz, Biomedical Imaging Group, Swiss Federal Institute of Technology Lausanneįigure 1. An ImageJ plugin for the recursive alignment of a stack of images.
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